WebTetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline).. Tetracycline-controlled gene expression is based upon the mechanism of resistance to tetracycline antibiotic treatment found in gram … WebPlasmid pCAG-Tet-on Advanced -BGH-PA from Dr. Yuet Wai Kan's lab contains the insert rtTA and BGH-pA and is published in Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19467 …
pTet-On Advanced Vector Information - Takara Bio
WebpVgRxR pOPRSVI pOPI3CAT pCMVLacI pSwitch pGene v5-His B pcDNA4/TO/Myc-His A pcDNA4/TO/Myc-His B pcDNA4/TO/Myc-His C pcDNA4/TO/Myc-His/LacZ pcDNA6/TR pTet-On pTet-Off pTRE2 pTRE2 hygro pTK-hyg pRevTet-On pRevTet-Off pRevTRE pTet on advanced pTRE Tight pTRE Tight Luciferase pCMV-Tet3G pTRE3G pTRE3G-Luc pNI v2 … WebVector for expressing tTS, which represses expression from Tet-responsive promoters in the absence of doxycycline. ... pTet-On-Advanced. pcDNA3.1 nV5-DEST. pCMV-Tag 4C. pFC14A HaloTag CMV. pTet-tTS. pcDNA3.1 V5-His A. pCMV-Tag 5A. pFC14K HaloTag CMV. pTetOne. pcDNA3.1 V5-His B. pCMV-Tag 5B. pFC15A HaloTag CMVd1. choosing the right glasses frames
pTet-On-Advanced vector map and sequence - novoprolabs.com
WebApr 23, 2013 · The T vector containing 2399 bp fragment and the pTet-On Advanced Vector plasmid were respectively digested by Cla I and BamH I restriction enzyme (New England Bio Labs, Mass, USA). The resulted fragments were separated by 1.0% agarose gel electrophoresis and retrieved by DNA retrieve KIT (Tiangen, China). The two Cla I and … WebThe Tet-On Advanced transactivator. The pTet-On Advanced vector constitutively expresses the tetracycline-controlled transcriptional transactivator, Tet-On Advanced (Urlinger . et … Web(Empty Backbone) Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles Depositing Lab. Eva Frickel. Publication. Fisch et al EMBO J. 2024 Jul 1;38(13):e100926. doi: 10.15252/embj.2024100926. ... The plasmid can be used in conjunction with pTet-CTRL … great america website