WebbStable down-regulation of CXCR4 was achieved by transduction of a lentiviral vector with short hairpin RNA (shRNA) for CXCR4 (shRNA-CXCR4), generated by GenePharma (GenePharma, Shanghai, China), to knock down the expression of CXCR4. Complementary DNA oligonucleotides were then subcloned into the pLV-IRES-GFP backbone to generate … Webb10 apr. 2024 · The lentivirus shRNA knockdown vector system is a highly efficient method for stably knocking down expression of a target gene in a wide variety of mammalian …
The combination of the tubulin binding small molecule PTC596 …
WebbpLV-RNAi Protocols – Design shRNA Designing oligonucleotide encoding shRNA (sense-loop-antisense) sequence. The features of single-strand oligonucleotide encoding … Webb28 jan. 2024 · In this chapter, we introduce an inducible pLV-shRNA vector system (Biosettia, San Diego, CA) for gene silencing. pLV-shRNA is a lentivirus-based shRNA vector that can mediate efficient introduction of shRNA into cells through lentiviral transduction especially in some difficult-to-transfect model systems. quest diagnostics burns rd palm beach gardens
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Webb19 maj 2024 · Likewise, the PSEN2 shRNA plasmid was assembled and linked to pLV-hU6-hef1a-P2A (Syngentech) and human PSEN2. pLV-hef1a-P2A-WPRE-CMV or pLV-hU6 … WebbpLV-eGFP was a gift from Pantelis Tsoulfas (Addgene plasmid # 36083 ; http://n2t.net/addgene:36083 ; RRID:Addgene_36083) For your References section: A multifunctional neurotrophin with reduced affinity to p75NTR … WebbWith an shRNA insert, the length of the TRC2-pLKO-puro plasmid is 7,518 bp, as indicated in the vector map below. Without an shRNA insert, the TRC2-pLKO-puro vector has a length of 7,484 bp. The sequence file provided is the empty TRC2-pLKO-puro vector (7,484 bp). TRC2-pLKO-puro sequence (without shRNA insert) shipping pets across us