Polymerases interact with the phosphate backbone and the minor groove of the DNA, so their interactions do not depend on the specific nucleotide sequence. The binding is largely mediated by electrostatic interactions between the DNA and the "thumb" and "palm" domains of the metaphorically hand-shaped DNA polymerase molecule. When the polymerase advances along the DNA sequence after adding a nucleotide, the interactions with the minor groove dissociate b… Web16 mei 2006 · An MCM 4,6,7 subcomplex possesses weak helicase activity in vitro (10, 11) that is improved in the presence of forked DNA substrates and ssDNA-binding protein . It …
The DExH protein NPH-II is a processive and directional motor for ...
Web10 jul. 2024 · We use our results to analyze velocity and processivity data from single molecule experiments on the superfamily-4 ring helicase T7, and establish quantitatively … WebSingle-molecule optical trap measurements of the unwinding activity of a single XPD helicase in the presence of RPA2 reveal a mechanism in which XPD interconverts between two states with different processivities and transient RPA1 interactions stabilize the more processive state, activating a latent “processivity switch” in XPD. Expand buick 1979
(PDF) A Conserved Helicase Processivity Factor Is Needed for ...
Web25 aug. 2006 · UvrD exhibits modest processivity as a DNA helicase (40–50 bp) (53– 55) making this protein an interesting choice for the helicase responsible for the unwinding event in MMR. UvrD is also the helicase responsible for the unwinding event associated with excision repair ( 56 – 59 ), which requires unwinding of a short 12–13 base long … WebIntegrative and conjugative elements (ICEs) are agents of horizontal gene transfer and have major roles in evolution and acquisition of new traits, including antibiotic … WebWe have asked whether a DNA helicase can unwind DNA contained within both isolated native chromatin and reconstituted chromatin containing regularly spaced arrays of nucleosome cores on a linear tandem repeat sequence. We find that Escherichia coli recBCD enzyme is capable of unwinding these DNA substrates and displacing the … buick 1973